ABSTRACT
Pseudomonas mendocina P2d grew in sodium benzoate at as high as 1% concentration and formed a quinonoid compound, identified as ortho-benzoquinone, that rendered the medium orange to wine-red in colour. The quinone was not metabilised further by the organism. Sodium benzoate was converted to catechol, which was a central metabolite forming ortho-benzoquinone and 2- hydroxymuconic semialdehyde (2-HMS) via. meta ring cleavage pathway.
Subject(s)
Aldehydes/metabolism , Benzoquinones/metabolism , Catechols/metabolism , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Models, Chemical , Pseudomonas mendocina/metabolism , Sodium Benzoate/metabolism , Time FactorsABSTRACT
Reduction of p-benzoquinone to hydroquinol has been studied using Saccharomyces cerevisae and S.uvarum. Maximum conversion of p-Benzoquinone (p-BQ) to Hydroquinol (HQ) at a substrate concentration of 6%. S. cerevisae was found to be better than S. uvarum. No further conversion did take place after 60 hr. Optical innoculam density was found to be 5-6% (v/v). The pH optima was observed at pH value 5.50. No significant improvement could be observed by doping acetone, which solubilizes p-BQ, to the medium.